Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus

Year: 2009

Authors: De Stefano L., Rea I., De Tommasi E., Rendina I., Rotiroti L., Giocondo M., Longobardi S., Armenante A., Giardina P.

Autors Affiliation: Unit of Naples-Institute for Microelectronics and Microsystems, National Council of Research, Via P. Castellino 111, 80131, Naples, Italy;
Dept. of Organic Chemistry and Biochemistry, University of Naples “Federico II”, Via Cintia 4, 80126, Naples, Italy;
LICRYL – INFM-CNR, Via P. Bucci, Cubo 33/B, 87036 Arcavacata di Rende, Cosenza, Italy

Abstract: A crystalline silicon surface can be made biocompatible and chemically stable by a self-assembled biofilm of proteins, the hydrophobins (HFBs) purified from the fungus Pleurotus ostreatus. The protein-modified silicon surface shows an improvement in wettability and is suitable for immobilization of other proteins. Two different proteins were successfully immobilized on the HFBs-coated chips: the bovine serum albumin and an enzyme, a laccase, which retains its catalytic activity even when bound on the chip. Variable-angle spectroscopic ellipsometry (VASE), water contact angle (WCA), and fluorescence measurements demonstrated that the proposed approach in silicon surface bioactivation is a feasible strategy for the fabrication of a new class of hybrid devices.

Journal/Review: EUROPEAN PHYSICAL JOURNAL E

Volume: 30 (2)      Pages from: 181  to: 185

KeyWords: Properties of solutions of macromolecule; Other topics in areas of applied and int;
DOI: 10.1140/epje/i2009-10481-y

Citations: 27
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