Protein unfolding and refolding under force: Methodologies for nanomechanics

Year: 2005

Authors: Samorì B., Zuccheri G., Baschieri P.

Autors Affiliation: Univ Bologna, Dept Biochem G Morruzzi, I-40126 Bologna, Italy;Univ Bologna, Natl Inst Phys Matter, I-40126 Bologna, Italy; CNR, Ist Proc Chim Fis, I-56010 Pisa, Italy

Abstract: An increasing number of inter- and intramoleculor interactions can nowadays be probed using single-molecule manipulation techniques. Protein unfolding and refolding is the most representative-though complex-of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We con also compare some of the physical aspects behind the two most frequently used single-molecule manipulation instrumentations: optical tweezers and atomic force microscopes.

Journal/Review: CHEMPHYSCHEM

Volume: 6 (1)      Pages from: 29  to: 34

KeyWords: AFM; protein folding;
DOI: 10.1002/cphc.200400343

Citations: 34
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