Combination of two-photon fluorescence microscopy and label-free near-infrared reflectance: A new complementary approach for brain imaging
Authors: Costantini I., Mascaro A.L.A., Margoni E., Iannello G., Bria A., Sacconi L., Pavone F.S.
Autors Affiliation: European Laboratory for Non-Linear Spectroscopy, University of Florence, Via Nello Carrara 1, Sesto Fiorentino, 50019, Italy; National Research Council, National Institute of Optics, Largo Fermi 6, Florence, 50125, Italy; Department of Engineering, Università Campus Bio-Medico di Roma, Rome, 00128, Italy; Department of Electrical and Information Engineering, University of Cassino, Italy; University of Florence, Department of Physics and Astronomy, Via Sansone 1, Sesto Fiorentino, 50019, Italy
Abstract: This work describes a complimentary approach that combines the reflected NIR light with two-photon fluorescence microscopy. A Thy1-GFPM mouse model will be studied; both fixed samples and live animals will be imaged.
More Information: Optical Tomography and Spectroscopy 2016KeyWords: Brain mapping; Fluorescence; Fluorescence microscopy; Neuroimaging; Optical tomography; Photons, Brain imaging; Label free; Mouse models; Near infra-red reflectances; Two-photon fluorescence microscopy, Infrared devicesDOI: 10.1364/CANCER.2016.JW3A.23