Piezoelectric Nanoparticle-Assisted Wireless Neuronal Stimulation
Authors: Marino, A; Arai, S; Hou, YY; Sinibaldi, E; Pellegrino, M; Chang, YT; Mazzolai, B; Mattoli, V; Suzuki, M; Ciofani, G
Autors Affiliation: Ist Italiano Tecnol, Ctr MicrobioRobot, I-56025 Pisa, Italy; Scuola Super Sant Anna, Biorobot Inst, I-56025 Pisa, Italy; WASEDA Biosci Res Inst Singapore WABIOS, Singapore 138667, Singapore; Univ Pisa, Dipartimento Ric Traslaz & Nuove Tecnol Med & Chi, I-56126 Pisa, Italy; Natl Univ Singapore, Dept Chem, MedChem Program, Inst Life Sci, Singapore 117543, Singapore; ASTAR, Singapore Bioimaging Consortium, Lab Bioimaging Probe Dev, Singapore 138667, Singapore; Waseda Univ, Org Univ Res Initiat, Shinjuku Ku, Tokyo 1620041, Japan
Abstract: Tetragonal barium titanate nanoparticles (BTNPs) have been exploited as nanotransducers owing to their piezoelectric properties, in order to provide indirect electrical stimulation to SH-SY5Y neuron-like cells. Following application of ultrasounds to cells treated with BTNPs, fluorescence imaging of ion dynamics revealed that the synergic stimulation is able to elicit a significant cellular response in terms of calcium and sodium fluxes; moreover, tests with appropriate blockers demonstrated that voltage-gated membrane channels are activated. The hypothesis of piezoelectric stimulation of neuron-like cells was supported by lack of cellular response in the presence of cubic nonpiezoelectric BTNPs, and further corroborated by a simple electroelastic model of a BTNP subjected to ultrasounds, according to which the generated voltage is compatible with the values required for the activation of voltage-sensitive channels.
Journal/Review: ACS NANO
Volume: 9 (7) Pages from: 7678 to: 7689
KeyWords: barium titanate nanoparticles; ultrasounds; piezoelectricity; SH-SY5Y cells; calcium imagingDOI: 10.1021/acsnano.5b03162Citations: 158data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2023-02-05References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here