Nonlinear laser imaging of skin lesions

Year: 2008

Authors: Cicchi R., Sestini S., De Giorgi V., Massi D., Lotti T., Pavone F.S.

Autors Affiliation: L.E.N.S. and Department of Physics, University of Florence, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy;
Department of Dermatology, University of Florence, Via della Pergola 58, 50121, Florence, Italy;
Department of Human Pathology and Oncology, University of Florence, Viale G. B. Morgagni 85, 50134, Florence, Italy

Abstract: We investigated different kinds of human ex-vivo skin samples by combined two-photon intrinsic fluorescence (TPE), second-harmonic generation microscopy (SHG), fluorescence lifetime imaging microscopy (FLIM), and multispectral two-photon emission detection (MTPE). Morphological and spectroscopic differences were found between healthy and pathological skin samples, including tumors. In particular, we examined tissue samples from normal and pathological scar tissue (keloid), and skin tumors, including basal cell carcinoma (BCC), and malignant melanoma (MM). By using combined TPE-SHG microscopy we investigated morphological features of different skin regions. Further comparative analysis of healthy skin and neoplastic samples was performed using FLIM, and MTPE. Finally, we demonstrated the use of methyl-aminolevulinate as a contrast agent to increase the contrast in BCC border detection. The results obtained represent further support for in-vivo noninvasive imaging of diseased skin.

Journal/Review: JOURNAL OF BIOPHOTONICS

Volume: 1 (1)      Pages from: 62  to: 73

KeyWords: Basal cell carcinoma; Fluorescence lifetime imaging microscopy; Intrinsic fluorescence; Lifetime; Morphological features; Multiphotons; Non-invasive imaging; Second-harmonic, Dermatology; Photons; Tumors, Fluorescence, diagnostic agent, adult; aged; article; basal cell carcinoma; confocal microscopy; female; histology; human; keloid; laser; male; melanoma; methodology; middle aged; multiphoton microscopy; pathology; skin; skin disease; skin tumor, Adult; Aged; Carcinoma, Basal Cell; Female; Humans; Keloid; Lasers; Male; Melanoma; Microscopy, Confocal; Microscopy, Fluorescence, Multiphoton; Middle Aged; Skin; Skin Diseases; Skin Neoplasms
DOI: 10.1002/jbio.200710003

Citations: 65
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