Position control and optical manipulation for nanotechnology applications
Authors: Capitanio M., Cicchi R., Pavone F.S.
Autors Affiliation: LENS, Via Nello Carrara 1, 50019 Sesto Fiorentino, Firenze, Italy and Dipartimento di Biologia Animale e Genetica “Leo Pardi”, Via Romana 17/19 50125 Firenze, Italy;
LENS, Via Nello Carrara 1, 50019 Sesto Fiorentino, Firenze, Italy and Dipartimento di Fisica-Università degli Studi di Firenze, Via Giovanni Sansone 1, 50019 Sesto Fiorentino, Firenze, Italy
Abstract: During the last decade, great advances have been made concerning the construction and manipulation of nanostructures. As a consequence, nanometer stability and control of the sample position have became crucial points. For this purpose, we have built an optical microscope with high mechanic stability and we have implemented a feedback system in order to compensate thermal drifts. We demonstrate the system stability to be within one nanometer, with a control on the sample position of some micrometers, along the three spatial directions. The sample can be manipulated optically by means of a multiple optical tweezers setup and its displacements measured with a 3D position detector. We discuss and characterize the system properties thoroughly. We finally test the apparatus on a bio-molecular system constituted by a single myosin motor interacting with an actin filament.
Journal/Review: EUROPEAN PHYSICAL JOURNAL B
Volume: 46 (1) Pages from: 1 to: 8
KeyWords: Control systems; Feedback; Fluorescence; Micrometers; Nanostructured materials; Nanotechnology; Optical microscopy; Photodetectors; Proteins; Thermal noise, Feedback systems; Optical trapping; Single molecule manipulation of other biological molecules; Single molecule manipulation of proteins, Position controlDOI: 10.1140/epjb/e2005-00232-2Citations: 56data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2023-01-29References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here