Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

Year: 2005

Authors: Cicchi R., Pavone F.S., Massi D., Sampson D.D.

Autors Affiliation: LENS & Department of Physics, University of Florence Via G. Sansone 1, 50019 Sesto Fiorentino, Florence, Italy;
Department of Human Pathology and Oncology, University of Florence Viale G.B.Morgagni 85, 50134 Florence, Italy;
Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia

Abstract: We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging. (C) 2005 Optical Society of America.

Journal/Review: OPTICS EXPRESS

Volume: 13 (7)      Pages from: 2337  to: 2344

KeyWords: Acetic acid; Concentration (process); Fluorescence; Glycols; Light propagation; Medical imaging; Multiphoton processes; Photons; Propylene; Second harmonic generation; Tissue, Hyperosmotic agents; Light propagation in tissues; Optical clearing agents (OCA); Two-Photon microscopy, Skin
DOI: 10.1364/OPEX.13.002337

Citations: 106
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