Multiphoton multifocal microscopy exploiting a diffractive optical element
Authors: Sacconi L., Froner E., Antolini R., Taghizadeh M.R., Choudhury A., Pavone FS.
Autors Affiliation: University of Trento, Via Sommarive 14, 38050 Povo (Trento), Italy; Department of Physics, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK; European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019 Sesto Fiorentino (Florence), Italy; Department of Physics, University of Florence, via Sansone 1, 50019 Sesto Fiorentino (Florence), Italy; Istituto Nazionale per la Fisica della Materia, Sezione di Firenze, via Sansone 1, 50019 Sesto Fiorentino (Florence), Italy
Abstract: Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds. (C) 2003 Optical Society of America.
Journal/Review: OPTICS LETTERS
Volume: 28 (20) Pages from: 1918 to: 1920
KeyWords: Imaging systems; Microscopy; OptoelectronicsDOI: 10.1364/OL.28.001918Citations: 115data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2022-09-25References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here