New techniques in linear and non-linear laser optics in muscle research

Year: 2006

Authors: Vanzi F., Capitanio M., Sacconi L., Stringari C., Cicchi R., Canepari M., Maffei M., Piroddi N., Poggesi C., Nucciotti V., Linari M., Piazzesi G., Tesi C., Antolini R., Lombardi V., Bottinelli R., Pavone FS.

Autors Affiliation: LENS (European Laboratory for Non-linear Spectroscopy), University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Firenze, Italy
Human Physiology Unit, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy
DBAG, University of Florence, Via G. Sansone 1, 50019 Sesto Fiorentino, Firenze, Italy
Department of Physics, University of Florence, Via G. Sansone 1, 50019 Sesto Fiorentino, Firenze, Italy
Department of Physiology, University of Florence, Sesto Fiorentino, Firenze, Italy
Department of Physics, University of Trento, Trento, Italy

Abstract: This review proposes a brief summary of two applications of lasers to muscle research. The first application (laser tweezers), is now a well-established technique in the field, adopted by several laboratories in the world and producing a constant stream of original data, fundamental for our improved understanding of muscle contraction at the level of detail that only single molecule measurements can provide. As an example of the power of this technique, here we focus on some recent results, revealing the performance of the working stroke in at least two distinct steps also in skeletal muscle myosin. A second laser-based technique described here is second-harmonic generation; the application of this technique to muscle research is very recent. We describe the main results obtained thus far in this area and the potentially remarkable impact that this technology may have in muscle research.


Volume: 27 (5-7)      Pages from: 469  to: 479

KeyWords: actin; muscle protein; myosin; nanoparticle, biomechanics; biotechnology; cytology; data analysis; device; imaging; laboratory; laser; linear system; measurement; medical research; methodology; microscopy; molecular dynamics; muscle; muscle cell; muscle contraction; muscle function; nonhuman; nonlinear system; optics; priority journal; protein conformation; protein protein interaction; review; skeletal muscle, Animals; Lasers; Microscopy; Microscopy, Polarization; Muscle Contraction; Muscles; Myosins; Optical Tweezers
DOI: 10.1007/s10974-006-9084-3

Citations: 34
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