Arrhythmia susceptibility in a rat model of acute atrial dilation
Authors: Scardigli M., Cannazzaro S., Coppini R., Crocini C., Yan P., Loew LM., Sartiani L., Cerbai E., Pavone FS., Sacconi L., Ferrantini C.
Autors Affiliation: European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, FI, Italy; CNR, Natl Inst Opt, I-50125 Florence, Italy; Univ Florence, Dept NeuroFarBa, Div Pharmacol, I-50139 Florence, Italy; Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA; Univ Colorado, BioFrontiers Inst, Boulder, CO 80309 USA; Univ Connecticut, Sch Med, RD Berlin Ctr Cell Anal & Modeling, Farmington, CT 06030 USA; Univ Florence, Dept Phys & Astron, I-50019 Sesto Fiorentino, FI, Italy; Univ Florence, Dept Expt & Clin Med, Div Physiol, I-50134 Florence, Italy
Abstract: Atrial fibrillation (AF) is the most common cardiac arrhythmia, associated with an increased risk of stroke and heart failure. Acute AF occurs in response to sudden increases of atrial hemodynamic load, leading to atrial stretch. The mechanisms of stretch-induced AF were investigated in large mammals with controversial results. We optimized an approach to monitor rat atrial electrical activity using a red-shifted voltage sensitive dye (VSD). The methodology includes cauterization of the main ventricular coronary arteries, allowing improved atrial staining by the VSD and appropriate atrial perfusion for long experiments. Next, we developed a rat model of acute biatrial dilation (ABD) through the insertion of latex balloons into both atria, which could be inflated with controlled volumes. A chronic model of atrial dilation (spontaneous hypertensive rats; SHR) was used for comparison. ABD was performed on atria from healthy Wistar-Kyoto (WKY) rats (WKY-ABD). The atria were characterized in terms of arrhythmias susceptibility, action potential duration and conduction velocity. The occurrence of arrhythmias in WKY-ABD was significantly higher compared to non-dilated WKY atria. In WKY-ABD we found a reduction of conduction velocity, similar to that observed in SHR atria, while action potential duration was unchanged. Low-dose caffeine was used to introduce a drop of CV in WKY atria (WKY-caff), quantitatively similar to the one observed after ABD, but no increased arrhythmia susceptibility was observed with caffeine only. In conclusion, CV decrease is not sufficient to promote arrhythmias; enlargement of atrial surface is essential to create a substrate for acute reentry-based arrhythmias. (C) 2019 Published by Elsevier Ltd.
Journal/Review: PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
Volume: 154 Pages from: 21 to: 29
KeyWords: CONTRACTION-EXCITATION FEEDBACK; REFRACTORY PERIOD; HEART-FAILURE; FIBRILLATION PROMOTION; PULMONARY VEINS; STRETCH; CONDUCTION; VULNERABILITY; DILATATION; PRESSUREDOI: 10.1016/j.pbiomolbio.2019.08.012Citations: 2data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2021-12-05References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here