Scientific Results

Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues

Year: 2020

Authors: Lagarto JL., Shcheslavskiy V., Pavone FS., Cicchi R.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy;
European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy; Becker & Hickl GmbH, Nunsdorfer Ring 7-9, 12277 Berlin, Germany: Privolzhskiy Medical Research University, 603005 Nizhny Novgorod, Russia; Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy

Abstract: We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.

Journal/Review: OPTICS LETTERS

Volume: 45 (8)      Pages from: 2247  to: 2250

KeyWords: Raman spectroscopy, lifetime spectroscopy, real-time imaging
DOI: 10.1364/OL.389300

Citations: 1
data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2021-11-21
References taken from IsiWeb of Knowledge: (subscribers only)
Connecting to view paper tab on IsiWeb: Click here
Connecting to view citations from IsiWeb: Click here

gdpr-image
This site uses cookies. If you decide to continue browsing we consider that you accept their use. For more information about cookies and how to delete them please read our Info Policy on cookies use.
Read more