Real‐time fiber‐based fluorescence lifetime imaging with synchronous external illumination: A new path for clinical translation
Authors: Lagarto JL., Shcheslavskiy V., Pavone FS., Cicchi R.
Autors Affiliation: 1National Institute of Optics, National Research Council (INO-CNR), Sesto Fiorentino, Italy; European Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, Italy; Becker & Hickl GmbH, Berlin, Germany; Department of Physics, University of Florence, Sesto Fiorentino, Italy
Abstract: Time-correlated single photon counting is the “gold-standard” method for fluorescence lifetime measurements and has demonstrated potential for clinical deployment. However, the translation of the technology into clinic is hindered by the use of ultrasensitive detectors, which make the fluorescence acquisition impractical with bright lighting conditions such as in clinical settings. We address this limitation by interleaving periodic fluorescence detection with synchronous out-of-phase externally modulated light source, thus guaranteeing specimen illumination and a fluorescence signal free from bright background light upon temporal separation. Fluorescence lifetime maps are generated in real-time from single-point measurements by tracking a reference beam and using the phasor approach. We demonstrate the feasibility and practicality of this technique in a number of biological
specimens, including real-time mapping of degraded articular cartilage. This method is compatible and can be integrated with existing clinical microscopic, endoscopic and robotic modalities, thus offering a new pathway towards label-free diagnostics and surgical guidance in a number of clinical applications.
Journal/Review: JOURNAL OF BIOPHOTONICS
Volume: 12 Pages from: e201960119-1 to: e201960119-14
KeyWords: fiber optics, fluorescence lifetime imaging, phasors, surgical guidance, TCSPC, tissue
spectroscopyDOI: 10.1002/jbio.201960119Citations: 3data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2021-03-07References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here