Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes
Authors: Sanzari I., Dinelli F., Humphrey E.J., Terracciano C. M., Prodromakis T.
Autors Affiliation: School of Engineering, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom; Consiglio Nazionale delle Ricerche (CNR), INO UOS ‘A. Gozzini’, Area della Ricerca di Pisa – S. Cataldo, via Moruzzi 1, I-56124 Pisa, Italy; National Heart and Lung Institute, Imperial College London, London, United Kingdom; Electronic Materials and Devices Research Group, Zepler Institute for Photonics and Nanoelectronics, University of Southampton, Southampton SO17 1BJ, United Kingdom
Abstract: In cardiac tissue engineering (TE), in vitro models are essential for the study of healthy and pathological heart tissues in order to understand the underpinning mechanisms. In this scenario, scaﬀolds are platforms that can realistically mimic the natural architecture of the heart, and they add biorealism to in vitro models. This paper reports a novel and robust technique to fabricate cardiovascular-mimetic scaﬀolds based on Parylene C and Polydimethylsiloxane (PDMS). Parylene C is employed as a mask material for inducing hybrid and non-hybrid micropatterns to the PDMS layer. Hybrid architectures present striped hydrophobic/hydrophilic surfaces, whereas non-hybrid scaﬀolds only corrugated topographies. Herein, we demonstrate that wavy features on PDMS can be obtained at the micro- and nanoscale and that PDMS can be integrated into the microfabrication process without changing its intrinsic physical properties. A study of the eﬀects of these scaﬀolds on the growth of Neonatal Rat Ventricular Myocytes (NRVMs) cultures reveals that cell alignment occurs only for the case of hybrid architectures made of hydrophilic PDMS and hydrophobic Parylene C.
Journal/Review: MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume: 103 Pages from: 109783-1 to: 109783-11