Imaging of human urothelial carcinoma samples using multimodal multiphoton microscopy

Year: 2017

Authors: Baria E., Barone A., Nesi G., Pavone F.S., Cicchi R.

Autors Affiliation: European Laboratory for Non-Linear Spectroscopy, Via Nello Carrara 1, Sesto Fiorentino, 50019, Italy; Department of Surgery and Translational Medicine, University of Florence, Largo Brambilla 3, Florence, 50134, Italy; National Institute of Optics, National Research Council, Largo Enrico Fermi 6, Florence, 50125, Italy; Department of Physics, University of Florence, Via Sansone 1, Sesto Fiorentino, 50019, Italy

Abstract: We combined Second Harmonic Generation and Two-Photon Fluorescence for imaging ex vivo tissue sections of human bladder affected by urothelial carcinoma. We studied different grades of the tumor, and compared them to healthy bladder mucosa.

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KeyWords: Nonlinear optics, Ex-vivo; Multi-modal; Multi-photon microscopy; Two photon fluorescence; Urothelial carcinoma, Microscopic examination