Design and construction of platinum-zinc oxide grafted honey-chitosan nanofibers loaded with hesperidin and study its antibacterial and wound healing effects

Year: 2025

Authors: Talib SM., Haider AJ., Al-Musawi S., Alnasraui AHF., Talib LM., Taha BA., Alnayli RS., Ezzat M., Salame C., Sheykhhasan M.

Autors Affiliation: Univ Technol Baghdad, Appl Sci Dept, Laser Sci & Technol Branch, Baghdad, Iraq; Al Qasim Green Univ, Coll Food Sci, Babylon 51013, Iraq; Al Qasim Green Univ, Coll Biotechnol, Babylon 51013, Iraq; Univ Baghdad, Coll Sci Women, Baghdad, Iraq; Univ Kebangsaan Malaysia, Fac Engn & Built Environm, Dept Elect Elect & Syst Engn, UKM Photon Technol Res Grp, Bangi 43600, Malaysia; Al Mustaqbal Univ, Coll Hlth & Med Tech, Radiol Tech Dept, Babylon 51001, Iraq; CNR, INO, Intense Laser Irradiat Lab ILIL, Via Moruzzi 1, I-56124 Pisa, Italy; Beni Suef Univ, Laser Inst Res & Applicat LIRA, Bani Suwayf 62511, Egypt; European Acad Sustainable Dev EURACA, 11 Rue Rempart St Thiebault, F-57000 Metz, France; Hamadan Univ Med Sci, Res Ctr Mol Med, Hamadan, Iran.

Abstract: Wound healing studies take several directions, like nanoformulated mats for effective therapeutic research. This study aims to design and synthesize nanofibrous mats to accelerate the wound healing process. This work presents the synthesis and characterization of core-shell zinc oxide (ZnO) and platinum (Pt) nanoparticles, synthesized via pulsed laser ablation, and their incorporation into honey/tripolyphosphate (TPP)/chitosan (HTCs) nanofibers with hesperidin (HD) for wound dressing applications. ZnO and Pt nanoparticles exhibited characteristic absorbance peaks at 350 nm and 210 nm, respectively, with structural analysis revealing a hexagonal wurtzite structure for ZnO and a cubic lattice for Pt. The transmission electron microscopy (TEM) and scanning electron microscopy (SEM) confirmed spherical morphology, with hydrodynamic radii of 30.59 nm for ZnO, 31.19 nm for Pt, and 61.18 nm for Pt-ZnO. Molecular docking models were created to examine the relationship between Hesperidin and 1SK3 protein to identify potential binding sites. These nanofibers demonstrated enhanced antibacterial activity, particularly the HTCs-HD/Pt-ZnO formulation, which showed significant inhibition zone 21.4 mm against K. rhinoscleromatis and19.8 mm against S. pyogenes. MTT assays showed cell viability of 61 % at 100 % extract concentration for HTCs-HD/Pt-ZnO NPs, compared to 82 % for HTCs and 80 % for HTCs-HD, confirming acceptable biocompatibility. Morphological analysis revealed that the addition of after 1-h cross-linking, suggesting reduced exudate uptake but improved structural integrity. Overall, these re

Journal/Review: MATERIALS CHEMISTRY AND PHYSICS

Volume: 345      Pages from: 131249-1  to: 131249-18

More Information: This research was funded by Malaysia Ministry of Higher Education (MOHE) , Malaysia, through the Fundamental Research Grant Scheme (FRGS) under the grant FRGS/1/2021/TK0/UKM/02/17 and Dana Pecutan from the Faculty of Engineering and Built Environment, Uni-versiti Kebangsaan Malaysia. The authors would like to extend gratitude toward Photonics Technology Research Group, Lab, Pusat Kejuruteraan Elektronik Dan Komunikasi Terkehadapan (PAKET) , Faculty of Engi-neering and Build Environment, Universiti Kebangsaan Malaysia, as well as the University of Technology, Baghdad, Iraq.
KeyWords: Antibacterial activity; Nanofibers; Hesperidin; Laser ablation in liquid; Pt-ZnO core-shell; Molecular docking; Wound dressing
DOI: 10.1016/j.matchemphys.2025.131249