Multiphoton morpho-functional imaging of healthy colon mucosa, adenomatous polyp and adenocarcinoma
Anno: 2013
Autori: Cicchi R., Sturiale A., Nesi G., Kapsokalyvas D., Alemanno G., Tonelli F., Pavone F.S.
Affiliazione autori: National Institute of Optics, National Research Council (INO-CNR), Largo E. Fermi 6, Florence, I-50125, Italy; European Laboratory for Non-Linear Spectroscopy (LENS), Via N. Carrara 1, Sesto Fiorentino, I-50019, Italy; Department of Clinical Physiopathology, Surgical Unit, University of Florence, Florence, I-50100, Italy; Division of Human Pathology and Oncology, Department of Surgical and Medical Critical Care, University of Florence, Florence, I-50100, Italy; Department of Physics, University of Florence, Via Giovanni Sansone 1, 50019, Sesto Fiorentino, Italy
Abstract: Two-photon spectral resolved imaging was used to image fresh human biopsies of colon tissue and to characterize healthy colon mucosa, adenomatous polyp and adenocarcinoma by means of a morpho-functional analysis. Morphological examination, performed using endogenous tissue fluorescence, discriminated adenomatous and adenocarcinoma tissues from normal mucosa in terms of cellular asymmetry and nucleus-to-cytoplasm ratio. Good agreement was found between multiphoton images and histological examination performed on the same samples. Further characterization, performed by means of spectral-resolved analysis of NADH and FAD fluorescence, demonstrated an altered metabolic activity in both adenomatous and adenocarcinoma tissues compared to healthy mucosa. This morpho-functional approach may represent a powerful method to be used in combination with endoscopy for in vivo optical diagnosis of colon cancer and may be extended to other tissues. (C) 2013 Optical Society of America
Giornale/Rivista: BIOMEDICAL OPTICS EXPRESS
Volume: 4 (7) Da Pagina: 1204 A: 1213
Maggiori informazioni: The research leading to these results has received funding: from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreements 228334 and 284464; from the Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX; from the Ente Cassa di Risparmio di Firenze (private foundation).Parole chiavi: Cytology; Fluorescence; Histology, Colon tissues; Functional approach; Functional imaging; Histological examination; Metabolic activity; Multiphotons; Optical Diagnosis; Spectral resolved, TissueDOI: 10.1364/BOE.4.001204Citazioni: 33dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-04-14Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui