Combining lateral shear interferometry with digital holography for quantitative phase microscopy
Anno: 2006
Autori: Ferraro P., Alfieri D., De Nicola S., De Petrocellis L., Finizio A., Pierattini G.
Affiliazione autori: CNR – Istituto Nazionale di Ottica Applicata, Via Campi Flegrei, 34 80072 Pozzuoli (Na), Italy;
Istituto di Cibernetica “E.Caianiello” del CNR Via Campi Flegrei, 34 80072 Pozzuoli (Na), Italy
Abstract: By combining the concept of Lateral Shear Interferometry (LSI) with Digital Holography we demonstrate that quantitative phase microscopy (QPM) can be used for investigation in different field of applications. The proposed approach gives some important advantages compared to other methods used for QPM. The method is a true single image QPM approach. In fact by using the digital shear of the reconstructed phase map in the image plane the defocus aberration introduced by the microscope objective can efficiently removed. In addition in most cases the unwrapping procedure can be avoided greatly simplifying the phase-map recovery for quantitative measurement. Numerical lateral shear of the reconstructed wave front in the image plane makes it possible to retrieve the derivative of the wave front. In analogy with the standard procedure usually applied in optical testing by means of LSI, the wave front can be reconstructed.
Giornale/Rivista: PROCEEDINGS OF SPIE
Volume: 6341 Da Pagina: 634115 A: 634115
Parole chiavi: Holography; Coherence Imaging; Digital Image Processing; Biology; DOI: 10.1117/12.695355Citazioni: 1dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-05-12Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui