Supercontinuum source tuned by an on-axis monochromator for fluorescence lifetime imaging

Anno: 2010

Autori: Mercatelli R., Soria S., Molesini G., Bianco F., Righini G., Quercioli F.

Affiliazione autori: CNR – Istituto di Fisica Applicata “N. Carrara”, Sesto Fiorentino; CNR – Istituto dei Sistemi Complessi, Sesto Fiorentino; CNR – Istituto Nazionale di Ottica, Firenze

Abstract: We report on the application of an optically tunable coherent white light source, based on the supercontinuum generation from microstructured optical fibres, to fluorescence lifetime imaging (FLIM) microscopy and Foerster resonance energy transfer (FRET). A prototype lens based on monotonic longitudinal chromatic aberration is used for tuning the supercontinuum wavelengths from 400 to 1000 nm and acts as an axial monochromator, suitable for fibre delivery in confocal microscopy. (C) 2010 Optical Society of America

Giornale/Rivista: OPTICS EXPRESS

Volume: 18 (19)      Da Pagina: 20505  A: 20511

Maggiori informazioni: The European NoE \”Photonics for Life\” (P4L) is acknowledged. F. Quercioli and R. Mercatelli gratefully acknowledge the funding of Ente Cassa di Risparmio of Florence, Italy. HEK cells were generously provided by Prof. A. Arcangeli. Useful discussions with G. Nunzi Conti and S. Pelli are also gratefully acknowledged. F. Bianco is now at the University of Trento, Italy.
Parole chiavi: Confocal microscopy; Energy transfer; Light sources; Monochromators; Optical fibers, Chromatic aberration; Fluorescence lifetime imaging; Foerster resonance energy transfers; Microstructured; Optical fibre; Supercontinuum; Supercontinuum generations; Supercontinuum sources; White light sources; Fluorescence, article; equipment; Equipment design; Fluorescence microscopy; Fluorescence resonance energy transfer; Illumination; Instrumentation; Equipment Design; Equipment Failure Analysis; Fluorescence Resonance Energy Transfer; Lighting; Microscopy; Fluorescence
DOI: 10.1364/OE.18.020505

Citazioni: 16
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