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Cardiac Imaging Group

National Institute of Optics  - European Laboratory for Non-linear Spectroscopy


The Cardiac Imaging Group is developing innovative imaging methodologies to increase the understanding of cardiac physiology. For instance, we recentlly developed an ultrafast random access multi-photon (RAMP) microscope that, in combination with a custom synthesized voltage-sensitive dye, is used to simultaneously measure action potentials and intracellular calcium transients at multiple sites within the sarcolemma of cardiac cells with submillisecond temporal and submicrometer spatial resolution. An ultrafast wide- field macroscope was also developed to optically map action potential propagation with a redshifted voltage sensitive dye in whole mouse hearts. The macroscope was implemented with a random-access scanning head capable of drawing arbitrarily-chosen stimulation patterns with submillisecond temporal resolution allowing precise epicardial activation of Channelrhodopsin2 (ChR2). We employed this optical system in the setting of ventricular tachycardia to optimize mechanistic-based, multi-barrier defibrillation patterns.

NEWS



CONTACT



Email : sacconi@lens.unifi.it

Phone Off: +39 055 4572451

Phone Lab: +39 055 4572018 - 2019

Skype : leonardo.sacconi

Address : European Laboratory for Non-linear Spectroscopy
Via Nello Carrara 1, 50019 Sesto Fiorentino (FI), Italy